Volkswagen, Wink
A preppy-type guy drives down the usual S-curve road for a car commercial, but drives through a section of fuzzy tree stuff and gets a piece in his eye.
He then arrives at a stoplight, occasionally blinking because of the object in his eye. He turns to find a group of Hell's Angels on motorcycles next to him and inadvertently winks at a scowling member of the gang.
When the gang member sees the man apparently wink at him, he plays along and winks back, to the driver's nervous dismay. The motorcycle guy laughs maniacally and rides away.
With $40 million behind the overall campaign (including this commercial), it was widely seen nationwide.